Claro Scientific, LLC
Claro Scientific, LLC

SpectraWave™

The SpectraWave system has three main components, sample preparation, light signal capture, and communications systems.

Sample Preparation Systems. The optical characterization of complex fluids, particularly those of a biological origin, demands a comprehensive approach for sample preparation and analysis. SpectraWave sample preparation is based on advanced microfluidic systems used for cell concentration and fractionation.

In maximizing system flexibility, Claro was aware that the nature of the sample will differ for each application (e.g. sensitivity to shear for biological materials) and the sampling environment (e.g. static, at/in line). This defined the need for adaptable sample preparation components. SpectraWave’s sample preparation components are being configured on a modular basis. Throughout prototype development, Claro has demonstrated a combination of knowledge and expertise in customizing sample preparation components to maximize SpectraWave’s utility in diverse R&D, laboratory and process environments.

Unlike other diagnostic systems, SpectraWave sample preparation is reagentless and fully automated. This eliminates the costs associated with handing and storing reagents. Since samples processed by SpectraWave require no manual preparation, processing, the need for a highly trained personnel is eliminated. Any person can insert a sample into a SpectraWave system and order the test to be performed. 

 Prototype SpectraWave unit

 

Signal Capture Systems.  SpectraWave uses a combination of optical methods to simultaneously capture several light measurements based on their dominant interaction mode.  On their own, each has advantages and limitations that often create hurdles in the interpretation of measurements from a single method.  Combined they supply unique and confirmatory information used to identify, characterize and quantify a sample with a high level of sensitivity and specificity, even at low concentrations.  Claro’s integrated approach (1) overcomes interpretation hurdles associated with the individual measurements and (2) enhances the amount of actionable information that can be extracted from the combined measurements.

Spectroscopic Method Sample Characteristics
Transmission/Absorption Size and size distribution, shape, chemical composition, particle count
Angular Scattering Size and size distribution, shape, structure, particle count
Forward Scattering Size, shape, orientation
Fluorescence Chemical composition, conformation, internal structure, fluorophore interactions
Polarization Size, shape, internal structure
Reflectance Size, chemical composition, particle count
Raman Chemical composition

 

SpectraWave systems use spectrophotometers capable of measuring the entire ultraviolet-visible-near infrared spectrum in milliseconds for transmission, absorption, fluorescence, polarization and reflectance measurements.  These components can be easily expanded to capture any other desired region of the electromagnetic spectrum as needed for analysis.  Multiple spectrometer configurations and combinations of different measurement modes are used simultaneously in order to enhance the informational outcome of the analysis.  Systems can capture static and/or dynamic (flow) modes of measurement.  The advanced features of the optical components include a large dynamic range (resolution: particle size/wavelength; from >190 to <1000 nm), small sampling times (<0.01 sec), and a large instrumental signal to noise ratio (>99.9%).  As a result, optical measurements are suitable for in situ, at-line and in-line applications.

Communication Systems. For certain applications, SpectraWave will be equipped with cellular, Wi-Fi, Ethernet and Bluetooth capabilities.  Users will be able to drive single or multiple SpectraWave systems by using any Bluetooth enabled smart phone, tablet, laptop or computer system.   SpectraWave software will send sample information to SpectraNet using Internet, satellite or cellular communications and users will receive sample results in minutes.